Chromatography column

ABSTRACT

A chromatography column having a longitudinal axis and comprising a column wall with a first end and a second end, a first end plate assembly removably connectable to said first end of the column wall, a second end plate assembly removably connectable to said second end of the column wall, wherein said first end plate assembly, said column wall and said second end plate assembly are arranged along the longitudinal axis of the column wherein the column wall, and/or first end plate assembly and/or second end plate assembly is/are rotatable about an axis of rotation wherein said axis of rotation is parallel to the longitudinal axis of said column and positioned outside the column.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. patent application Ser. No.12/250,569 filed Oct. 14, 2008, now U.S. Pat. No. 8,778,188, which is adivisional of U.S. patent application Ser. No. 11/183,522 filed Jul. 18,2005, U.S. Pat. No. 7,452,464, which claims priority to applicationnumber 0424259.0 filed in Great Britain on Nov. 2, 2004.

FIELD OF THE INVENTION

The present invention relates to chromatography columns of the typementioned in the preambles of the independent claims.

BACKGROUND OF INVENTION

Chromatography columns may be used in industrial process to purifyprocess liquids and separate substances of interest from processliquids. Prior art chromatography columns comprise a column wall in theform of hollow column tube which is connected to a removable upper endplate assembly and a removable lower end plate assembly. One end plateassembly is provided with a process fluid inlet arrangement, typicallycomprising an inlet pipe and an inlet valve and the other end plateassembly is provided with a process fluid outlet arrangement, typicallycomprising an outlet pipe and an outlet valve. Each end of the columntube is usually provided in the interior of the column with a removabledistribution system. These inlet and outlet distribution systems may beattached to the respective end plate assembly or the upper distributionsystem may be arranged to be movable towards or away from the end plateassembly. During use, the space in the column between the distributionsystems is usually filled with a chromatography medium. If necessary aretaining mesh may be provided between each distribution system and themedia. The inlet distribution system is intended to distribute incomingfluid evenly over the surface of the media at the inlet end of thecolumn while the outlet distribution system is intended to collect fluidevenly from the surface of the media at the outlet end of the columnSuch a column may weigh several tonnes. Typically the end plateassemblies are bolted to flanges provided at each end of the columnAlternatively the end plate assemblies may be connected by longitudinaltie bars. Seals are usually provided between the end plates assemblies,inlet and outlet pipes, distribution systems and valves in order toprevent leakage. Columns are typically provided with legs to raise thelower end plate assembly off the ground in order to provide access tothe lower end plate assembly, the inlet or outlet pipe and the valvearrangement. When such a column requires maintenance to, or cleaning of,internal components, such as the valves, seals, distribution systems,etc. lifting gear such as a crane is necessary to lift the upper endplate assembly off the column tube and the column tube off the lower endplate assembly.

SUMMARY OF THE INVENTION

According to the present invention, at least some of the problems withthe prior art are solved by means of a device having the featurespresent in the characterising part of claim 1.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1a shows a partial cross-sectional view of a first embodiment of achromatography column in accordance with the present invention.

FIG. 1b shows a plane view from above of the column of FIG. 1 a.

FIG. 2 shows a view corresponding to that of FIG. 1a wherein the firstend plate and distribution system have been raised above the columnwall.

FIG. 3 shows a view corresponding to that of FIG. 2a wherein the firstend plate and distribution system have been raised above the columnwall, and the column wall has been raised above the second end plate.

FIG. 4 shows a view corresponding to that of FIG. 3 wherein the firstend plate and distribution system have been raised above the columnwall, and the column wall has been raised above the second end plate androtated out from a position between the first and second end plates.

FIG. 5 shows the column after the feed system has be relowered in orderto allow easy access to it.

FIG. 6 shows a perspective view of a second embodiment of the presentinvention having only 2 extendable legs.

FIG. 7 shows a side view of the column of FIG. 6 with the cylinder wallin a position corresponding to that of FIG. 4.

FIGS. 8-14 shows lateral views and cross-sections of a third embodimentof the present invention.

DETAILED DESCRIPTION OF THE INVENTION

FIGS. 1-5 show a first embodiment of a chromatography column 1 inaccordance with the present invention. Column 1 comprises a hollowcolumn wall 3, which in this embodiment is in the form of a cylinder 5,with a first open end 7 and a second open end 9. Each open end 7, 9 isprovided with an outward projecting circumferential flange 11,respectively 13, arranged perpendicular to the longitudinal axis L ofthe column wall 3. A first end plate assembly 15 is removably fastenedto flange 11 and a second end plate assembly 17 is removably fastened toflange 13. In this embodiment of the present invention the column isintended to be used in the down-flow mode, i.e.

the first end plate assembly 15 is intended to be used to feed fluids tothe column 1 and the second end plate assembly is intended to be used toremove fluids from the column, however the present invention is equallyapplicable to columns using the up-flow mode. In use, column wall 3 maybe occupied by a chromatography medium 19. First end plate assembly 15is provided with the usual fluid feed system 21 (also called “movableadapter”) comprising one or more valves, distribution channels,distribution nets, nozzles, connectors, etc which are well-known in theart and which are referenced collectively under the reference number 21.In this example of a column, first end plate assembly 15 comprises firstend plate 23 which is removably fastenable to flange 11, and which has acentral opening 25 through which a hollow shaft 27 is movable in thelongitudinal direction of the column by a motor 29. In use, hollow shaft27 projects into the column cavity 31 and supports the fluid feedsystem. Motor and drive assembly 29 can be used to move the movableadapter 21 towards and away from first end plate 23 and thereby adjustthe depth of movable adapter 21 in the column 1. Second end plateassembly 17 is provided with the usual fluid collecting system 33comprising one or more valves, distribution channels, distribution nets,nozzles, connectors, etc. which are well-known in the art and which arereferenced collectively under the reference number 33.

Column 1 is supported in frame 35. Frame 35 comprises feet 37 in contactwith the ground. Feet 37 support three extendable legs 39, 41, 43 whichextend upwards and are each provided with a substantially horizontal arm45, 47, 49. Arms 45-49 pass over the column 1 are attached to end plate23. Extendable legs 39-43 provided with lifting means such as jacks orrams arranged to be able to lift arms 45-49 and the first end plate 23sufficiently high enough so that when movable adapter 21 has been raisedto its closest possible position to the underside of first end plate 23it is possible to raise the first end plate assembly 15 so that none ofit is inside the column wall 3.

Column wall 3 is attached to leg 41 by a pair of arms 51, 53 which aremounted on a sleeve 55 on leg 41. Sleeve 55 is rotatable around leg 41and is also movable along the longitudinal axis “1” of leg 41.

FIGS. 1a and 1b show the chromatography column in the normal operatingcondition with first and second end plates sealed against the ends ofcolumn wall.

FIGS. 2-4 shows stages in rearranging the column allow access to thedistribution systems and end plates.

In FIG. 2, the column is shown after it has been emptied of media andfluids, movable adapter 21 has been raised by motor until the movableadapter 21 is close to the bottom of first end plate assembly and thenfirst end plate has been unfasten from the top of column wall and raisedby means of extendable legs 39-41 so that the lowest part of movableadapter 21 is above the top of cylinder wall.

In FIG. 3, the column is shown after column wall has been detached fromsecond end plate assembly and sleeve 55 has been raised along thelongitudinal axis “1” of 41 so that none of movable adapter 21 or secondend plate assembly is inside the cylinder wall.

FIG. 4 shows the column after the cylinder wall has been rotated aboutaxis “1” so that the column wall is no longer positioned between thefirst and second end plate assemblies.

FIG. 5 shows the column after the movable adapter has be relowered inorder to allow easy access to it.

FIG. 6 shows a perspective view of a second embodiment of the presentinvention having only 2 extendable legs.

FIG. 7 shows a side view of the column of FIG. 6 with the cylinder wallin a position corresponding to that of FIG. 4.

FIGS. 8-14 show lateral views of a third embodiment of a column inaccordance with the present invention. In this embodiment of a column,the column has two-legs 339, 341 and is adapted so that the movableadapter lifting means such as motor and drive assembly 329 for themovable adapter 321 is able to be used for extending the legs 339, 341.Thus no jacks or rams are needed to extend the column legs 339, 341. Inorder to achieve this upper end of the column wall 303 is provided withat least one movable adapter locking pin 371 able to be moved to andfrom locking engagement with a locking bore 373 arranged on movableadapter 321. Preferably the locking pin 371 and locking bore 373 arearranged so that locking pin 371 is able to be moved into locking bore373 when the movable adapter has been raised to substantially itsuppermost position. When locking pin(s) is/are in locking engagementwith movable adapter 321 relative movement between movable adapter 321and the column wall 303 is prevented.

Legs 339 and 341 are each provided with an upper telescopic shaftlocking pin 375 able to be moved to and from a position where itprevents movement of telescopic shafts 379 which are slidably arrangedin the upper portions of legs 339, 341.

Preferably the locking pins 375 are arranged so that locking pins 375are able to be moved into locking position when the telescopic shafts379 has been raised to substantially their most extended positions,thereby locking telescopic shafts 379 in the raised position.

Leg 341, which supports column wall arms 351, 353, is provided with alocking hole 381 into which a locking pin 383 mounted on arm 353 may bemoved into and out of locking engagement. Preferably the locking pin 383and locking hole 381 are arranged so that locking pin 383 is able to bemoved into locking hole 381 when the column wall 303 has been raised toa position where it is no longer in contact with second end plateassembly 317.

The following description and FIGS. 8-14 describe and illustrate thesteps of a method in accordance with the present invention forrearranging the components of a column in order to allow the column wall303 to be rotated to a position where it is no longer between the columnlegs 339, 341.

FIG. 8 shows a column 301 in its normal operating position with themovable adapter 321 in a possible working position inside column wall303 and telescopic shafts 379 retracted.

FIG. 9 shows a first step in the method in which the movable adapterlifting means 329 has raised the movable adapter 321 to substantiallyits uppermost position and movable adapter locking pin 371 has been slidinto engagement with locking bore 373 thus preventing further relativemovement between movable adapter 321 and column wall 303.

FIG. 10 shows the next stage in the method in which the removablefastening means such as bolts 385 for releasably fastening first endplate assembly 315 to the upper end of column wall 303 and bolts 387 forreleasably fastening second end plate assembly 317 to the lower end ofcolumn wall 303 have been unfastened and removed.

FIG. 11 shows the next stage in the method in which movable adapterlifting means 329 has been actuated in the way which during normaloperation would cause the movable adapter to be moved downwards.However, as movable adapter 329 is locked by locking pin 371 and lockingbore 373 to column wall, it is not possible for movable adapter 329 todescend—instead first end plate assembly 315 and all the columncomponents rigidly attached to it such as telescopic shafts 379 andmovable adapter lifting means 329 are raised. This movement is continueduntil telescopic shafts 379 have been extended sufficiently far so as toallow telescopic shaft locking pins 375 to be moved into position whichprevent the telescopic shafts 379 being lowered.

FIG. 12 shows the following stage in the method after movable adapterlifting means 329 has been actuated in the way which during normaloperation would cause the movable adapter to be moved upwards. Asmovable adapter 321 is attached to column wall 303 this has causedcolumn wall to be lifted off second end plate assembly 317. The movementhas been continued until locking pin 383 has become aligned with lockinghole 381 at which point locking pin 383 has been slid into locking hole381. Column wall 303 is now unable to be lowered as any downwardmovement is prevented by the interaction of locking pin 383 on arm 353with leg 341.

FIG. 13 shows a subsequent stage in the method in which movable adapterlocking pin 371 has been moved out of engagement with locking bore 373thereby allowing movable adapter to move relative to column wall 303.Movable adapter lifting means 329 has been actuated to lift movableadapter 321 so that none of it is inside column wall 303.

FIG. 14 shows a further stage in the method in which arms 351, 353supporting column wall 303 have been rotated around leg 341, therebymoving column wall 303 out from between first and second end plateassemblies 315, 317.

While examples of embodiments of the present invention have beenillustrated with columns using movable adapters, it is conceivable toadapt the present invention to columns which do not use movableadapters. In such cases lifting means need to be arranged to lift thefirst (upper) end plate assembly and column wall from the second (lower)end plate assembly, after which the column wall is prevented from beingraised (e.g. by locking its supporting arms to legs in analogy withabove), the fastenings between the first end plate assembly and columnwall are released and the first endplate assembly raised until it isabove the column wall which then may be rotated to the side.Analogously, it is also possible to adapt the present invention tocolumns which do not have an upper end plate assembly. In such cases theadapter must be provided with releasable locking means which can be usedto lock it to the column wall in order to allow it to be used to raisethe column wall off the lower end-plate.

In a further embodiment of the present invention in addition to thecylinder wall being arranged to be able to rotate about axis “1”, one ormore end plates are arranged to be able to rotate about axis “1”.

In a further embodiment of the present invention, in addition to, orinstead of, being rotatably mounted on a column leg, the arms connectingthe column wall to the column leg are made telescopic in thesubstantially horizontal direction, thereby enabling the column wall tobe moved in a straight line out from between the end plate assemblies.

While the invention has been illustrated by examples in which thevertical movements of column components are achieved using poweredmeans, it is also possible to achieve these movements manually, forexample by using brute force or any manner of lifting devices—forexample, ropes and pulleys, winches, levers and jacks.

It is apparent that many modifications and variations of the inventionas hereinabove set forth may be made without departing from the spiritand scope thereof. The specific embodiments described are given by wayof example only, and the invention is limited only by the terms of theappended claims.

What is claimed is:
 1. A chromatography column, having a longitudinalaxis, comprising: a column wall with an upper first end and a lowersecond end; a first end plate assembly removably connectable to thefirst end of the column wall; a second end plate assembly removablyconnectable to the second end of the column wall; and a movable adapter,movable along the longitudinal axis from a position inside the columnwall to a position outside the column wall, wherein the first end plateassembly, the column wall, and the second end plate assembly arearranged along the longitudinal axis, and wherein the column wall isrotatable about an axis of rotation is parallel to the longitudinal axisof the column and positioned outside the column.
 2. The column of claim1, further comprising a movable adapter, movable along the longitudinalaxis of the column from a position inside the column to a positionoutside the column.
 3. The column of claim 2, wherein the movableadapter is provided with means for releasably locking the movableadapter to the column wall.
 4. The column of claim 1, further comprisinga movable adapter lifting means for lifting the movable adapter.
 5. Thecolumn of claim 1, further comprising lifting means for first end plateassembly, adapted for lifting the first end plate assembly above thecolumn wall.